1x tbst (Bio-Rad)

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Bio-Rad 1x tbst
1x Tbst, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 15810 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+tbst/Tween+20/pmc13034896-69-3-40
Average 98 stars, based on 15810 article reviews

1x tbst - by Bioz Stars, 2026-10

98/100 stars

Images

Milk:

Article Title: Cryo-EM reveals a right-handed double-helix dimer architecture of PCDH15 critical for mechanotransduction
Article Snippet: Eluted immunoprecipitated proteins and input lysates were separated on NovexTM WedgeWellTM 4–20% Tris-Glycine Mini Protein Gels (Life Technologies) and transferred onto PVDF membranes (Sigma) at 250 mA for 2.5 hours at 4°C using the Mini Blot Module (Bio-Rad) with Bio-Rad transfer buffer containing 20% methanol. .. Membranes were blocked for 1 hour in 5% Blotting-Grade Blocker Non-Fat Dry Milk (Bio-Rad) in 1X TBST (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.05% Tween-20), followed by overnight incubation at 4°C with primary antibodies (see below) in 5% milk in 1X TBST. ..

Incubation:

Article Title: Macrophage SH3 domain‑containing GRB2‑like protein B1 is required for Rab7‑mediated mitophagy in inflammation during sea water drowning acute lung injury.
Article Snippet: Primary antibodies: GAPDH (1:1,000; cat. no. ab8245; Abcam), HSP90 (1:1,000; cat. no. 13171‐1‐AP, Proteintech Group, Inc.), SH3GLB1 (1:1,000; cat. no. sc‐374146, Santa Cruz Biotechnology, Inc.), LC3 (1:1,000; cat. no. 12741T; CST Biological Reagents Co., Ltd.), P62 (1:1,000; cat. no. ab109012, Abcam), Rab7 (1:1,000; cat. no. A12308, ABclonal) and Parkin (1:1,000; cat. no. ab77924, Abcam). .. After washing with 1X TBST (containing 0.1% Tween‐20), membranes were incubated with HRP‐conjugated secondary antibodies (1:5,000, cat. no. 7074S/7076S; CST Biological Reagents Co., Ltd.) and bands were revealed using ECL‐HRP substrate (cat. no. WBAVDCH01; MilliporeSigma ) and quantified in Image Lab (Bio‐Rad Laboratories, Inc.). ..

Article Title: Macrophage SH3 domain-containing GRB2-like protein B1 is required for Rab7-mediated mitophagy in inflammation during sea water drowning acute lung injury
Article Snippet: Primary antibodies: GAPDH (1:1,000; cat. no. ab8245; Abcam), HSP90 (1:1,000; cat. no. 13171-1-AP, Proteintech Group, Inc.), SH3GLB1 (1:1,000; cat. no. sc-374146, Santa Cruz Biotechnology, Inc.), LC3 (1:1,000; cat. no. 12741T; CST Biological Reagents Co., Ltd.), P62 (1:1,000; cat. no. ab109012, Abcam), Rab7 (1:1,000; cat. no. A12308, ABclonal) and Parkin (1:1,000; cat. no. ab77924, Abcam). .. After washing with 1X TBST (containing 0.1% Tween-20), membranes were incubated with HRP-conjugated secondary antibodies (1:5,000, cat. no. 7074S/7076S; CST Biological Reagents Co., Ltd.) and bands were revealed using ECL-HRP substrate (cat. no. WBAVDCH01; MilliporeSigma) and quantified in Image Lab (Bio-Rad Laboratories, Inc.). ..

Article Title: Ribosome Molecular Aging Shapes Translation Dynamics
Article Snippet: Proteins were then transferred to 0.2 μm nitrocellulose membranes (BioRad, 1704158) on the Trans-Blot Turbo System (BioRad, 17001917). .. Membranes were blocked in blocking buffer containing 1X TBST (BioRad, #1706435) buffer and 5% bovine serum albumin (R&D systems, 5217) for 30 minutes, then incubated with primary antibodies overnight at 4°C in blocking buffer. ..

Imaging:

Article Title: Divergent condensates tune transcriptional responses during stress
Article Snippet: Cells on 6-well plates were washed 2x with ice-cold 1x PBS and lysed on ice in RIPA buffer (Thermo Fisher Scientific, 89901; ∼200 μL per well) supplemented with 1× protease and phosphatase inhibitors (Halt Protease Inhibitor Cocktail, 100×, 78430). .. Membranes were washed 3x with 1x TBST, subjected to chemiluminescent detection reagents (Biorad, 1705061 or 1705062) and imaged using a ChemiDoc imaging system. pAs used were as follows: rb-a-HSF1 (abcam, ab52757), rb-a-H3K27Ac (Invitrogen, 720096) and rb-a-HSF1-phospo S320 (abcam, ab76183). sAs used were as follows: goat-a-rb-HRP (CST, 7074S). ..

Saline:

Article Title: Glutamic Acid at position 168 is a constitutive activator of Tank Binding Kinase 1 catalytic function
Article Snippet: The loading samples were denatured at 80°C for 8 minutes, centrifuged at 5000x g for 1 minute, and the supernatant loaded onto a NuPAGE 4-12% precast gel (10338442, Fisher Scientific) for western blotting. .. The gels were then transferred onto TransBlot® TurboTM PVDF membranes (1704156, Bio-Rad) and blocked using 5% milk in 1x TBST (Tris-buffered saline, 0.05% Tween 20) for 1 hour. .. The blocked membranes were subjected to overnight incubation with the antibody preparation as follows: Anti-phospho TBK1 (D5C2, 5483S, Cell Signaling Technology), Anti-TBK1 (3013S, Cell Signaling Technology), Anti-phospho RAB7 (AB302494, Abcam), Anti-RAB7 (E907E, 95746S, Cell Signaling Technology), Anti-β-actin HRP (A3864, Sigma), and Anti-HA tag (H3663, Sigma).

Blocking Assay:

other:

Article Title: A hotspot phosphorylation site on SHP2 drives oncoprotein activation and drug resistance.
Article Snippet: Cells were lysed in Pierce RIPA lysis buffer (Thermo Scientific, Cat# PI87788) containing protease (Selleckchem, Cat# B14001) and phosphatase inhibitors (Selleckchem, Cat# B15001).

Review